Journal: Inflammation Research

Article Title: Differential regulation of 5-lipoxygenase and leukotriene-C4-synthase expression by IFNγ, IL-4 and IL-13 in human monocytes and macrophages from patients with atopic dermatitis

doi: 10.1007/s00011-025-02108-2

Figure Lengend Snippet: 5-Lipoxygenase (5-LO/ALOX5) protein expression is upregulated by IFNγ in human monocytes and macrophages. Monocytes ( A ) and macrophages ( C ) were cultured in growth chambers (chamber tek slides) with IFNγ for 24 h or left non-stimulated. Immunocytochemistry was performed using a mouse IgG2a-anti human 5-LO/ALOX5 antibody or an IgG2a isotype control (1 µg/ml). One representative example out of 6 independent experiments from 6 different donors is shown. Staining intensity was quantified by a digital computer-assisted method. The percentages of 5-LO/ALOX5 positive cells are shown in scatterplots for monocytes ( B ) and for macrophages ( D ). Data are shown as individual values with medians. Significant differences, as determined by Wilcoxon matched-pairs signed rank test are indicated as follows: * p < 0.05; B (n = 6); D (n = 6)

Article Snippet: Monocytes and macrophages were stimulated with IL-4 (20 ng/mL), IL-13 (15 ng/mL) or IFNγ (200 ng/mL) (all R&D Systems, Wiesbaden, Germany) for 24 h and 48 h for analyzing the mRNA expression and 24 h for analyzing the protein expression by immunocytochemistry.

Techniques: Expressing, Cell Culture, Immunocytochemistry, Control, Staining

Journal: Inflammation Research

Article Title: Differential regulation of 5-lipoxygenase and leukotriene-C4-synthase expression by IFNγ, IL-4 and IL-13 in human monocytes and macrophages from patients with atopic dermatitis

doi: 10.1007/s00011-025-02108-2

Figure Lengend Snippet: 5-Lipoxygenase (5-LO/ALOX5) mRNA expression is upregulated by IFNγ and downregulated by IL-4 and IL-13 in human monocytes and macrophages from atopic dermatitis (AD) patients. Human monocytes ( A , B ) and macrophages differentiated in the presence of M-CSF ( C , D ) obtained from healthy volunteers (HV) and from AD patients were stimulated with IFNγ, IL-13 or IL-4 for 24 and 48 h or left non-stimulated (NS). 5-LO/ALOX5 mRNA expression was detected by q-PCR and shown as target/reference (Tgt/Ref) ratio. Data are shown as individual values with medians. Significant differences, as determined by Friedman Dunn´s multiple comparison test (black straight bars) or by Kruskal-Wallis test (grey angled bars) are indicated as follows: * p < 0.05; ** p < 0.01; *** p < 0.001; A (n HV = 13, n AD = 15); B (n HV = 8, n AD = 6); C (n HV = 13, n AD = 18); D (n HV = 6, n AD = 6)

Article Snippet: Monocytes and macrophages were stimulated with IL-4 (20 ng/mL), IL-13 (15 ng/mL) or IFNγ (200 ng/mL) (all R&D Systems, Wiesbaden, Germany) for 24 h and 48 h for analyzing the mRNA expression and 24 h for analyzing the protein expression by immunocytochemistry.

Techniques: Expressing, Comparison

Journal: Inflammation Research

Article Title: Differential regulation of 5-lipoxygenase and leukotriene-C4-synthase expression by IFNγ, IL-4 and IL-13 in human monocytes and macrophages from patients with atopic dermatitis

doi: 10.1007/s00011-025-02108-2

Figure Lengend Snippet: Leukotriene-C4-synthase (LTC4S) mRNA expression is upregulated by IL-13 in human monocytes from healthy volunteers (HVs). Human monocytes ( A , B ) and macrophages differentiated in the presence of M-CSF ( C , D ) obtained from HVs and from atopic dermatitis (AD) patients were stimulated with IFNγ, IL-13 or IL-4 for 24 and 48 h or left non-stimulated (NS). LTC4S mRNA expression was detected by q-PCR and shown as target/reference (Tgt/Ref) ratio. Data are shown as individual values with medians. Significant differences, as determined by Friedman Dunn´s multiple comparison test are indicated as follows: * p < 0.05; ** p < 0.01; *** p < 0.001; A (n HV = 14, n AD = 14); B (n HV = 8, n AD = 6); C (n HV = 11, n AD = 17); D (n HV = 7, n AD = 6)

Article Snippet: Monocytes and macrophages were stimulated with IL-4 (20 ng/mL), IL-13 (15 ng/mL) or IFNγ (200 ng/mL) (all R&D Systems, Wiesbaden, Germany) for 24 h and 48 h for analyzing the mRNA expression and 24 h for analyzing the protein expression by immunocytochemistry.

Techniques: Expressing, Comparison